JPTRM Vol. 4 No. 2 (November 2016)
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Item Assessment for Sporicidal Activity of Two Types of Peroxygen/Silver-Based Disinfectants: A Comparative Study(Chitkara University Publications, 2016-11-02) Mostafa Essam EissaNumerous cases have been reported globally showing outbreaks, dissemination of infections and/or spoilage of medicinal products, food and other consumables that affects human life and may lead to death in some cases. One of the most critical measures of microbial contamination that should be taken into consideration is the use of proper disinfectant depending on the type of activities and the work load of the healthcare institution, subjects or media for microbial transfer and the affected population. The current study shows significant difference in sporicidal activity between two types of commercial peroxygen/Silver based disinfectants obtained from same manufacturer and delivered through same distributor at the same time. The peroxygen component in one of the disinfectant products is Hydrogen peroxide while in the other product is Peroxyacetic acid/Hydrogen Peroxide mixture. Preliminary rapid assessment of the disinfectants activity was required using the most resistance microbial form (bacterial spore) as a reference microorganism to challenge the biocidal products. Bacillus pumilus and B. subtilis spores were exposed to both disinfectants at three different concentrations levels covering the range recommended by the manufacturer: 1, 3 and 5% (v/v). The first formula did not exceed 0.5 logarithmic reduction(LR) even after 30 minutes. While the other product achieved more than two folds LR (more than 100 times reduction in microbial population) after ten minutes contact time. Appropriate initial screening of biocidal activity in commercial disinfectants market is critical step that should be performed by the healthcare facilities before practical application. Otherwise, inefficient control on bioburden may lead to devastating consequences on human health. An initial, non-laborious, time-saving and non-expensive screening test using the most resistance microorganisms is encouraged to be performed by healthcare facilities prior to practical application of disinfectant rather than reliance solely on random selection of biocidal agents using informational data without confirmatory experiments.Item Improvement of Learning and Memory of Mice by Plumbagin(Chitkara University Publications, 2016-11-02) D Dhingra; S ParshadRoots of Plumbago zeylanica have been reported to improve learning and memory of mice, but the effect of plumbagin on learning and memory of mice and the possible mechanisms for its effect on memory have not been explored till date. So the present study was designed to evaluate the effect of plumbagin on learning and memory of Swiss young male albino mice. Plumbagin (4, 8, 16 mg/kg, p.o.) and physostigmine (0.1 mg/kg,i.p.) per se were administered for 15 successive days to separate groups of mice. Behavioral parameters of learning and memory were recorded using Morris water maze. Acetylcholinesterase activity was estimated in brain of mice. Effect of plumbagin on scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.)-induced amnesia was also investigated. Locomotor activities of mice were also recorded. Plumbagin (8 and 16 mg/kg) and physostigmine significantly improved learning and memory of mice, as indicated by decrease in escape latency during training and increase in time spent in target quadrant of Morris water maze during retrieval. The drugs did not show any significant effect on locomotor activities of mice. Memory improving activity of plumbagin (16 mg/kg) was equivalent to physostigmine. Plumbagin significantly reversed scopolamine- and diazepam- induced amnesia in mice. Plumbagin and physostigmine also significantly reduced brain acetylcholinesterase activity of mice. In conclusion, plumbagin significantly improved memory of mice possibly through inhibition of brain acetylcholinesterase activity and through involvement of GABA-benzodiazepine pathway. Thus, plumbagin may be explored further for management of cognitive dysfunction.Item Toxicological Evaluation of Crude Alkaloid Fraction Isolated From Indian Folklore Plant Telosma Pallida (Roxb) wg Craib Root Using Probit Value Analysis(Chitkara University Publications, 2016-11-02) Punit R. Bhatt; Kajal B. Pandya; Navin R ShethPresent study first time reported the toxicological profile of Telosma pallida (TP) crude alkaloid fraction (CAF) isolated from the root of the climber plant by brine shrimp lethality test (BLT). Telosma pallida is a perennial herb found throughout the Junagadh district and surrounding. Previous studies showed the use of these alkaloids in the inhibition of thymidylate synthetase enzymes and cell growth. Brine shrimp toxicity study was carried out with Artemia salina Leach. In this assay, brine shrimp was hatched in sea salt water and allowed to contact with various concentrations of the crude alkaloid fractions. At 500μg/ml, highest mortality was found 74.44±0.35%whereas in the case of positive control, at the dose of 50μg/ml, 80.00±0.609% mortality was found. LD50 values for CAF and test control was found to be 89.12μg/ml and 12.59μg/ml, respectively. Further in-vitro and in-vivo studies may testify the anticancer potential of this plant.Item Analgesic Activity of some 1-Phenyl-3-aryl-5- (4-(butanoloxy) phenyl) 1H-pyrazoles(Chitkara University Publications, 2016-11-02) Anju Goyal; Sandeep JainA series of analgesic activity of synthesised 1 -Phenyl-3-aryl- 5-(4-(butanoloxy) phenyl) 1H-pyrazoles were synthesized from chalcones and screened for their in vitro analgesic activity. Chalcones i.e.,1-aryl-3- (4-hydroxyphenyl) prop-2-en-1-ones, 1 on reaction with phenyl hydrazine in presence of acetic acid and few drops of hydrochloric acid furnished the corresponding 1-phenyl-3-aryl-5-(4-hydroxyphenyl)-1H-pyrazoles 2 which on further reaction with 4-chloroalkanol yielded the title compounds 3. These compounds were characterized by CHN analyses, IR, mass and 1H NMR spectral data. All the novel synthesised 1-Phenyl-3-aryl-5-(4-(butanoloxy) phenyl) 1H-pyrazoles were evaluated pharmacologically for their analgesic activity and the title synthesised compounds exhibited significant results as compared to standard drug.Item Self-medication in Developing Countries a Systematic Review(Chitkara University Publications, 2016-11-02) Meena Parulekar; Nandakumar Mekoth; C.M. Ramesh; Ajit ParulekarSelf-medication is common in developing countries where it has both economic and social implications. On the one hand, it is viewed as a large component of self-care, which relies heavily on the consumer’s expertise in terms of experience of the consumer, when it comes to medication use. On the other hand, if not practiced correctly it can lead to multiple issues including abuse and drug resistance. Across surveys conducted in developing countries the reasons why people self-medicate has been studied, to understand the determinants of self-medication and to explain the influence of knowledge and information on self-medication practices. To understand the same a detailed systematic literature review based on survey findings on self-medication in developing countries was carried out. From a total of 52 survey articles, 25 surveys were selected for the present review. From the survey outcomes it was found that the cost, time and past experience with the medicine and symptom were key determinants while healthcare professionals emerged to be the primary sources of information and knowledge for self-medication practices. The prevalence of self-medication as a phenomenon is high and it has both potential benefits and associated risks with it. To ensure that the risks and benefits of medicine usage are known by consumers, we really need to look at and design suitable interventions to promote responsible self-medication and in turn, rational drug use in the developing world.