JPTRM Vol. 5 No. 1 (May 2017)

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https://demodspace.chitkara.edu.in/handle/123456789/134

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Browsing JPTRM Vol. 5 No. 1 (May 2017) by Author "Gulshan Bansal"

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    Standardization of Some Commercial Anti-diabetic Herbal Products containing Syzygiumcumini
    (Chitkara University Publications, 2017-05-05) Jasmeen Kaur; Munish Singla; Balraj Saini; Gulshan Bansal
    An isocratic HPLC method was developed for standardization of five commercially available products containing S. cumini seeds (DIABECON, MADHULENE, HYPONIDD, D-FIT and DIABEGON)using methylxanthoxylin (MXX)as marker. The method was validated in accordance with ICH Q2(R1) guidelines. The chromatographic separation of MXX was achieved on a C18 column by mobile phase composed of methanol and water (60:40%v/v) at a flow rate 0.5 mL/min. The eluent was detected at 280 nm. The method was linear over concentration of 1-200 μg/mL with correlation coefficient of 0.9999. The LOD and LOQ were 0.175 and 0.530 µg/mL, respectively. The method was precise (%RSD <0.31), accurate and robust for determination of MXX in herbal extracts. The content of MXX in the seed extract was found to be 0.0433%w/w while it was ranging from 0.026- 0.041%w/w in the products. The content of MXX was found to be equivalent to the pure seed extract only in DIABECON tablets and D-FIT soft gelatine capsules while it was found to be significantly less in the other formulations.
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    WHO Guided Real Time Stability Testing on Shankhpushpi Syrup
    (Chitkara University Publications, 2017-05-05) Astha Jain; Jasmeen Kaur; Nancy; Yogita Bansal; Balraj Saini; Gulshan Bansal
    Shankhpushpiis widely used herb in numerous CNS active Ayurvedic formulations, which are consumed by population of all age groups. The present study is designed to evaluate the stability of commercially available shankhpushpi syrup as per WHO guidelines. The study was conducted under long term conditions for 12 months on three different batches of the product. An HPLC-UV method was developed and validated to determine the marker (scopoletin) content in the stability samples. Peak purity was determined using HPLC-PDA analysis. Each stability sample was investigated for any change in physical, chemical and biological parameters with respect to control sample. The product was found to be stable in terms of physical parameters whereas significant change was observed in total phenolic content and content of scopoletin. The antioxidant, acetylcholine esterase inhibitory and antianxiety activities of the stability samples decreased with respect to control sample. Moreover, there was significant variation in chemical and biological parameters among the batches. The findings suggested that the product was unstable and further studies are required to be conducted in order to determine the shelf life of the product.