Browsing by Author "Sushma Devi"

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    Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract
    (Chitkara University Publications, 2024-04-20) Mamta Saini; Saurabh Gupta; Sushma Devi; Thakur Gurjeet singh
    Background: Green coffee beans are rich in bioactive compounds, particularly chlorogenic acids, which are known for their health benefits. Phytochemical screening and chromatographic techniques help identify and quantify these compounds. This research explores its phytochemical profile and antioxidant efficacy, contributing to its therapeutic relevance. Purpose: The current work was conducted to inspect the chromatographic and spectroscopic profile, phytochemical constituents, and antioxidant activity of the hydroethanolic extract of green coffee Arabica. Methods: The hydroethanolic extract of green coffee beans was extracted from shade-dried beans of green coffee Arabica using the Soxhlet extraction method. The bioactive compounds were identified using HPTLC. An analysis of the antioxidant profile of hydroethanolic GCBE and standards was carried out with the help of 2,2-Diphenyl-1-picryl-hydrazyl (DPPH), 2,2′-casino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS), nitric oxide radical assay, superoxide radical test, and lipid peroxidation. Results: The qualitative phytochemical analysis displayed that the hydroethanolic extract of green coffee beans consisted of carbohydrates, phenolic compounds, saponins, alkaloids, and flavonoids. The presence of chlorogenic acid and trigonelline (RF value 0.32 and 0.16) was confirmed by comparing it with standards (RF value 0.35 and 0.17), respectively. The total phenolic and flavonoid contents of GCBE were found to be 225.6 ± 0.37 & 42.53 ± 0.34 mg /g, respectively. The extract showed significant antioxidant activity. Conclusion: The hydroethanolic extract of green coffee beans had significant antioxidant activity and confirmed the presence of trigonelline, caffeic acid, chlorogenic acid, and quinic acid. It was concluded that due to the presence of a substantial amount of phenolic compounds, it showed considerable antioxidant activity.
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    Phytoconstituents in Breast Cancer Prevention
    (Chitkara University Publications, 2022-05-07) Shreya Sood; Sushma Devi; Thakur Gurjeet Singh; Amarjot Kaur; Vinita Singh; Prinsi Sahpuriya
    Background: In today’s developing nations, breast cancer (BC) is among the foremost risk factor for women. The causes of mortality are still unclear, yet they are frequently related to socioeconomic level and way of life. Numerous clinical therapies have been employed up to this point, i.e.,radiotherapy, hormonal treatment, targeted treatment,mastectomy, and treatment with drugs etc. Purpose: However, resistance development to chemotherapeutic drugs, radiation and hormonal therapy is one of the common problems for many of the patients with BC.Numerous studies have looked at the anti-cancer potential of natural products due to the fact that they are generally safe.Researchers’ interest is being drawn by phytochemicals from plants that are thought to be bioactive. In this review the formation and progression of breast cancer were also studied, along with the mode’s of activity and regulatory function of these phytoconstituents in major signalling networks within the cell. Conclusion:Phytoconstituents in Breast Cancer Prevention have potential future as well as the difficulties. So, need more study and instructions for experimental animals, medical research, and experimental in vitro and in vivo methods are critically evaluated.