Browsing by Author "Reecha Madaan"

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    Estimation of Isolated Triterpenoid – Ursolic Acid in Verbena officinalis L. Aerial Parts Using TLC Densitometry
    (Chitkara University Publications, 2014-11-30) Jashanjot Kaur; Deepak Kumar; Reecha Madaan; Suresh Kumar
    Verbena officinalis L. (Vervain; family-Verbenaceae),a traditionally used and medicinally promising plant, has been reported to contain triterpenoids as major class of phytoconstituents. But no work has ever been carried out on V. officinalis to isolate major chemical constituent(s), and to standardize plant material on the basis of isolated constituent(s) using TLC densitometry.The chloroform extract of V. officinalisaerial parts was prepared by extracting properly identified plant in a Soxhlet apparatus.Preliminary phytochemical screening of chloroform extract showed presence of triterpenoids. Column chromatography of chloroform extract using solvent systems viz., hexane:chloroform, chloroform and chloroform:methanol in different concentrationsyielded five fractions (F1- F5). Preliminary phytochemical screening of various fractions of chloroform extract revealed presence of triterpenoids in F2 and F3 fractions. Therefore, these fractions were further subjected to column chromatography. White colored crystals were obtained in SF1 sub-fraction separated from F2and was designated as VOC-1. Structure of VOC-1 was elucidated by IR and NMRspectral studies and was characterized as ursolic acid. Further, ursolic acid was quantified in V. officinalisaerial parts by validated TLC densitometric method. The content of ursolic acid was found to be 0.1580% in V. officinalis aerial parts.The linearity range, correlation coefficient, intra-day precision, inter-day precision, LOD, LOQ and accuracy were found to be 300-1800 ng, 0.997, 1.2% CV, 1.6% CV, 40 ng/spot, 130 ng/spot and 98.27% respectively.
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    Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants
    (Chitkara University Publications, 2014-05) Deepak Kumar; Anupam Jamwal; Reecha Madaan; Suresh Kumar
    Traditionally, aerial parts of Abies pindrow Royle (Himalayan Fir; Pinaceae); Abies webbiana Lindl. (Talispatra; Pinaceae); Cephalandra indica Naud. (Ivy Gourd; Cucurbitaceae) and roots of Calotropis gigantea (L.) Dryand (Giant Milkweed; Asclepiadaceae) have been used in the Indian systems of medicine for the treatment of various ailments. But no systematic phytochemical work has ever been carried out on these potential plants. Thus, it was planned to estimate total phenols and flavonoids content in methanol extract, ethyl acetate fraction (EAF) and remaining methanol extract (RME) of selected plants. Properly identified plants were defatted with petroleum ether, and then separately extracted in a Soxhlet apparatus with methanol. The methanol extract of each plant was partitioned by ethyl acetate solvent to obtain EAF of respective plant. The total phenols and flavonoids contents were estimated using standardized procedures. Quantitative determination of total phenols and total flavonoids was done using standard curve of gallic acid (linearity: 20 to 120 mg/ml; r2 = 0.995) and quercetin (linearity: 30 to 180 mg/ml; r2 = 0.997), respectively. EAF of selected plants contained higher content of phenols and flavonoids, where as lowest content was observed in RME of plants. The content of total phenols and flavonoids in selected plants were found to be in order of C. indica > A. webbiana > A. pindrow > C. gigantea. The available literature reveals that polyphenols have been reported to possess varied pharmacological activities. As selected Indian plants contain polyphenols as major class of phytoconstituents, it is suggested that these constituents may be responsible for their medicinal uses.
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    Screening of Antianxiety Activity of Habenaria Intermedia D. Don Fruits
    (Chitkara University Publications, 2017-05-05) Pawan Kumar; Reecha Madaan; Shabir Sidhu
    Habenaria intermedia D. Don (Vriddhi; Orchidaceae) has been traditionally used in the treatment of nervous disorders, skin disorders and asthma. The available pharmacological reports on H. intermedia reveal that the plant has not been screened for antianxiety activity. Thus, it was envisaged to subject H. intermedia for screening of antianxiety activity using elevated plus maze model. The crude extracts (n-hexane, chloroform, methanol and water extracts) of plant material were prepared successively in increasing order of polarity. The anxiolytic activity was assessed by comparing number of entries and average time spent by mice treated with test extracts (200 or 400 mg/kg, p.o.) in open arms of EPM with respect to control and standard drug, diazepam (2 mg/kg, p.o.). Significant antianxiety activity was observed in methanol extract with respect to control, whereas n-hexane, chloroform and water extracts did not exhibit antianxiety activity. It is further observed that antianxiety activity exhibited by the methanol extract was statistically not equivalent to the standard drug. Based on these observations, it is concluded that the methanol extract of H. intermedia exhibits mild anxiolytic activity.